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If you work in any "safety-sensitive" position with any Federal agency under the Department of Transportation, you must follow the rules and regulations for drug testing and alcohol testing; CFR Title 49 Part 40.  Whether you are a single owner-operator, or an employer with these positions, within your company, Twin Tiers Drug & Alcohol Testing, LLC will have you compliant in minutes; with a DOT drug & alcohol testing program that includes:

  • More Than 10,000 Collection Sites Available

  • Free Laboratory Account with Your Company on the CCF.

  • Pre-Employment Testing - Keep the Same Location You Use Now.

  • Randoms Management - Either in Our Consortium or Stand Alone.

  • On-Site Random Testing.

  • Drug Policy Implementation.

  • Reasonable Suspicion, Post-Accident Testing, and Support, 24/7.

  • DER Training, if Needed, with Lifetime Certificate - Train the Trainer Available as Well So You Can Train Your Supervisors!

  • MRO Mediation

  • Records Management

  • DOT Audit Assistance

  • And Much More!

We are a proud woman, family, and American-owned business.  We have the personal touch, with all the services you'd expect from a Fortune 500 company.  

View the entire CFR Title 49 Part 40


Did you know that over 77% of illicit drug users are employed in the United States?  That's almost 10% of America's workforce! More and more, Non-DOT regulated employers are adding drug & alcohol testing policies and procedures.  As a Non-DOT employer, you have a wide variety of options for drug testing.  Most states do not restrict the administration of a drug free workplace policy, and some offer workers compensation discounts to implement them.  Not sure which ones?  We do!  All of our Non-DOT testing can be used by employers, courts, families, substance abuse counselors, and more.


Non-DOT testing can include the following, in a number of combinations:

  • Urine Testing - expanded opiates available.

  • Hair Follicle Testing - 3 month window of detection for chronic drug use. Up to 17 substances available!

  • Oral Fluid Testing - 1-3 day window detection for current drug use.

  • Nail Bed Testing - 6 month window of detection!

  • PEth Blood Spot Testing - Alcohol biomarker that is much more reliable than urine EtG testing. Measures alcohol use over the previous 2-3 weeks

  • Evidential Breath Alcohol Testing - Immediate results from one of our Certified Breath Alcohol Technicians

ALL can be used for non-DOT regulated testing, and can show you a clearer picture of illicit drug use.  All results are either MRO Certified (Medical Review Officer) or F-ABFT Certified (Fellow of the American Board of Forensic Toxicology), and are admissible in a court of law. 


The future of Non-DOT testing is here, with i3ccf, Nexscreen, and Twin Tiers Drug & Alcohol Testing, LLC.  You HAVE to see this!

Nexscreen POCT


iPadfor i3ccf Soluton

Point of Care Testing with Nexscreen and i3ccf, will have results to you instantly!; and the elimination of the 5 part form is critical for businesses who like less paperwork to deal with.  There are many reasons to go paperless: less waste, less PHI (Protected Health Information) floating around, reducing your company's carbon footprint, and the process is much faster. Plus you'll see:

  • Error Reduction.

  • Instant Results.

  • Online Platform for Collection and Storage of Results.

  • MRO Confirmation for Non-Negative Specimens and MRO Adjudication Included.

  • eTesting in Our Office, or Yours.

  • Seamless Incorporation with Random Pools.

  • All One Low Price

Best of all, one of our Certified Specimen Collectors will collect and verify the results immediately; OR with training and support from Twin Tiers Drug & Alcohol Testing, LLC, you can do the testing yourself, at your place of business.  We would supply you with the software, the cups, the shipping supplies, and knowledge to complete the testing; and save even MORE money!  This all comes with 24/7/365 post-accident and reasonable suspicion support!


As you may know, Twin Tiers Drug & Alcohol Testing, LLC is the region's premiere drug testing location.  What you may not know is, when is comes to POCT, we are one of the only sites in two states that offer Quest ERO (Express Results Online) testing.  We also have other POCT devices and programs that can be tailored just for you.  

Twin Tiers Drug & Alcohol Testing, LLC also has the BEST pricing on bulk instant testing supplies.  If your company does your own, in-house testing program, we will meet or beat ANY company's price - GUARANTEED.  We offer instant test kits for urine, oral fluid, and alcohol testing.  Most are FDA approved and CLIA waived, with a variety of panels and adulterants available. Visit our store to see for yourself.

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